Preparation of YAC End Fragments from the Whitehead/MIT Mouse YAC Library pRML Vectors

Preparation of YAC end fragments from the whitehead/MIT mouse YAC library pRML vectors.

YAC Library Pooling Scheme for PCR-Based Screening

The PCR is a rapid method for screening a library of clones for the presence of clones containing an STS, Usually the library is divided into pools of clones, and the PCR is run on each pool. The problem we address here is to design efficient and robust pooling schemes for such PCR-based screening. Two questions are relevant: (1) Given an arbitrary unique sequence, how should one pool a library...

An automated method for DNA preparation from thousands of YAC clones.

We describe an automated method for the preparation of yeast genomic DNA capable of preparing thousands of DNAs in parallel from a YAC library. Briefly, the protocol involves four steps: (1) Yeast clones are grown in the wells of 96-well microtiter plates with filter (rather than plastic) well-bottoms, which are embedded in solid growth media; (2) These yeast cultures are resuspended and their ...

Generation of YAC transgenic mice by ICSI

Transgenic mice carrying an Xist-containing YAC.

The initiation of X-chromosome inactivation in female mammals is controlled by a key locus, the X-inactivation centre (Xic). The Xist gene, which maps to the candidate region for Xic and is expressed exclusively from the inactive X chromosome, is thought to be an essential component of the Xic. To test whether sequences spanning several hundred kilobases and including Xist from the Xic region a...